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Part:BBa_K1465307:Design

Designed by: iGEM-Team Bielefeld 2014   Group: iGEM14_Bielefeld-CeBiTec   (2014-10-09)


Isobutanol pathway with adhA from Lactococcus lactis under Ptac control


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 8429
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6916
    Illegal XhoI site found at 6196
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3579
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3165
    Illegal BsaI site found at 5982
    Illegal BsaI site found at 6239
    Illegal BsaI.rc site found at 1567
    Illegal BsaI.rc site found at 2161
    Illegal BsaI.rc site found at 3984


Design Notes

We used Gibson as the cloning method for the part BBa_K1465302. Afterwards we did several BioBrick Assemblies for combining the parts BBa_K731500, BBa_K1465302 and BBa_K1465301.

Source

References